The MFG-100-i allows for easy incorporation into an inverted microscope system, as shown here, where it can be used for a wide range of micro-mechanobiology studies. The inverted MFG-100 system enables single cell manipulation that can be combined with force sensing, and delivers a platform for in-vitro and in vivo fluid dynamics studies. Combined with high resolution optics, the MFG-100-i enables the investigation of cellular and sub-cellular mechanics as well as 5-DOF dextrous manipulation at sub-micron scales. Experimental exploration of anisotropic fluid dynamics with micron resolution at low Reynolds numbers is possible, and studies of various kinds of motion strategies with arbitrary field and gradient combinations can also be performed.